Product Description

This kit is suitable for the detection of Escherichia coli O157:H7 in food.

Detection principle

Based on real-time PCR technology, primers, fluorescent probes and other reagents for Escherichia coli O157:H7 specific genes were added to the samples for amplification. In the process of amplification, the fluorescent probe is bound to the target gene fragment, which can be decomposed by Taq enzyme and produce a fluorescent signal. At this time, the fluorescence quantitative PCR instrument can identify the fluorescence signal, and draw the corresponding real-time amplification curve according to its strength change, so as to determine whether Escherichia coli O157:H7 is detected.

Storage

-20ºC dark storage, valid for 12 months, avoid repeated freezing and thawing.

Sensitivity:
Minimum Test limit of 100 CFU/Test

Other required materials and applicable instruments:
Fluorescent quantitative PCR instrument (with fluorescent channel capable of detecting FAM label), high-speed centrifuge, pipette, pipette tip and centrifuge tube, etc