Usages:

 For isolation and cultivation of Listeria monocytogenes.

Principle:

Peptone provide carbon and nitrogen sources; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains osmotic equilibrium; glucose carbon source; agar as medium coagulant.

Formulation(per liter):

Trypsin digest of casein 10.0g

Heart trypsin digest 3.0g

Corn starch 1.0g

Pepsin digests meat 5.0g

Yeast extract powder 8.0g

Sodium chloride 5.0g

Glucose 0.5g

Mannitol 10.0g

Ferric ammonium citrate 0.5g

Esculin 0.8g

Lithium chloride 15.0g

Phenol Red 0.1g

Agar 15.0g

Final pH 7.2 ± 0.2

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.